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The Opacity input is used when using the Translucent Blend Mode, typically for Translucent, Additive, and Modulated materials. You can input a value between 0 and 1, where:
0.0 represents completely transparent.
1.0 represents fully opaque.
Opaque and masked blend modes also use Opacity when using one of the subsurface shading models.
Opacity is mostly used for Translucent, Additive, and Modulated Materials.